The compatibility of two species of Trichoderma spp. was tested in vitro and in soil with different fertilizing elements (N, P, K, Ca) added to increase concentrations in the solid potato-based medium. After 48 h of incubation, CaCl2, KNO3 and KH2PO4 at 400 mg/L showed an inhibitory effect on mycelial growth of Trichoderma harzianum strain (Tcomp) and Trichoderma viride strain (TV1) compared to controls varying respectively from 9.66 to 28.33 and 10.33 to 29.16 / 90.00 mm. For cons, the NH4NO3, (NH4) 2SO4 and CaSO4, 2H2O had a mean toxicity against the two strains tested, mycelial growth was ranged from 50.50 to 64.00 / 90 mm. After 7 days of incubation, both strains were able to produce conidia; their numbers have varied respectively from 6369.00 to 117833.20 / 245843.00 conidia / mm2 and 6793.00 to 67221.70 / 649635.00 conidia / mm2. The pH was slightly basic in the potato liquid medium before culturing and after the addition of the products at different concentrations (7.64-8.60). The pH became acid after culturing of the two strains; it varied between 3.13 and 5.85. Fresh weights were low in the presence of 50 mg / L of Calcium Chloride and medium for other products from 0.01 to 2.79 and 3.04 to 6.69 respectively compared to control 7.31 - 9.28 mg. Dry weights were low in the presence of the tested products compared to controls from 7.31 - 9.28 mg. In soil, both strains were able to grow on barley straw fragments at a concentration of 400 mg/L. The colonization percentage varied respectively between 63% and 94%. Two strains of Trichoderma showed substantial compatibility with the most part of fertilizers tested in vitro and a great capacity to keep the soil in the presence of different elements. Thus, the antipollution properties of Trichoderma spp. can be used in agriculture in soils with excess of nitrogen and phosphate. They also have the ability to increase the capacity of nutrient uptake by plants.

The effects of metal ions (Cu, Fe, Zn, Mg, Mn) on the life cycle and ability of two Trichoderma species to compete in the soil were searched. The strains of T. harzianum (Tcomp) and T. viride (TV1) presented a slowed mycelial growth (respectively 31 - 22 and 57.16 - 59.33 mm; 20 - 14.76 and 17.16 - 14.83 mm) on agar media supplemented with 200 and 400 mg/L copper and ferrous sulphate salts. By cons, in the presence of different concentrations of magnesium, zinc and manganese sulfates, mycelial growth varied between 66.66 and 90 mm compared to controls. Conidia production of the two strains was null in the presence of 400 mg/l of FeSO4 and reduced in the presence of different concentrations of other salts varying between 0.37 and 1.97 105 spores/mm2 compared to the controls, 4,50 105 spores/mm2 for Tcomp et 4,10 105 spores/mm2 for TV1 The neutral pH became acid after culturing of the two strains in the liquid medium with or without the test salts varying between 3.75 and 5.71, except in the presence of TV1 in media supplemented with FeSO4 and MgSO4. In addition, the two Trichoderma strains showed a very strong saprophytic soil activity exceeding 80% at a concentration of 100 mg/L of the salts tested. The ability of strains of Trichoderma to grow up and to show great saprophytic activity in the presence of metal ions and other soil fungi, suggests the possibility of using them for the remediation of contaminated soil.