The effects of metal ions (Cu, Fe, Zn, Mg, Mn) on the life cycle and ability of two Trichoderma species to compete in the soil were searched.
The strains of T. harzianum (Tcomp) and T. viride (TV1) presented a slowed mycelial growth (respectively 31 - 22 and 57.16 - 59.33 mm; 20 - 14.76 and 17.16 - 14.83 mm) on agar media supplemented with 200 and 400 mg/L copper and ferrous sulphate salts. By cons, in the presence of different concentrations of magnesium, zinc and manganese sulfates, mycelial growth varied between 66.66 and 90 mm compared to controls. Conidia production of the two strains was null in the presence of 400 mg/l of FeSO4 and reduced in the presence of different concentrations of other salts varying between 0.37 and 1.97 105 spores/mm2 compared to the controls, 4,50 105 spores/mm2 for Tcomp et 4,10 105 spores/mm2 for TV1
The neutral pH became acid after culturing of the two strains in the liquid medium with or without the test salts varying between 3.75 and 5.71, except in the presence of TV1 in media supplemented with FeSO4 and MgSO4. In addition, the two Trichoderma strains showed a very strong saprophytic soil activity exceeding 80% at a concentration of 100 mg/L of the salts tested.
The ability of strains of Trichoderma to grow up and to show great saprophytic activity in the presence of metal ions and other soil fungi, suggests the possibility of using them for the remediation of contaminated soil.