The objective of this work is to evaluate the content of total polyphenols in the leaves and stem of M. charantia. Three extraction methods (decoction, infusion and maceration) were used for a period of 60 minutes with a sample every 20 minutes. The conductivity and the coloring were evaluated on these different extracts. The total phenolic content of the extracts was estimated by Folin’s method. The color of the samples was measured based on the CIELAB color system using a colorimeter (KONICA MINOLTA-Japan). The results were processed with R software version 3.2.4 Revised (2018-03-16, R-70336) and Minitab 18 software. The results showed that decoction is the best method for extracting total polyphenols with 1.69 gAG.100.g-1 of extract, followed by infusion and maceration respectively 0.61 gAG.100.g-1 and 0.32 gAG.100.g-1. The analysis of the color of the extracts reveals that the intensity of the yellow color is more accentuated in decoction. The comparison of the content of phenolic compounds in the leaves and stems showed that the leaves and stems of M. charantia from the Fatick region are richer in total polyphenols than of M. charantia from the Dakar region.