Which focused on the contribution of blood safety in the prevention of HIV/AIDS contamination; it was used in the health zone of Gemena, we set ourselves the overall objective of observing and verifying whether the people involved in this chain of activities normally respect the application of the tests in relation to the biological qualification and the counseling before and after blood collection from donors. How they refer donors found to be HIV positive to a care service. The results from this study are as follows: We found that out of 30 subjects surveyed: - 50% have followed the training on biological diagnosis; - 60% have taken training in transfusion safety; - 73% of our respondents had not received training on voluntary counseling and testing, which meant that 83% thought only of the strategy of maintaining confidentiality, and not using DCIP; - It appears that 100% of our health facilities do not have donor files to allow rigorous selection; - 100% of our respondents use the correct biological qualification while applying immuno-haematologist and immuno-serological tests. Significant statistical relationships were observed between gender with VCT training; the professional category with training in biological diagnosis; seniority with health facilities; sex with keeping donor records and preventive measures; finally the level of study with training on transfusion safety.