Volume 74, Issue 2, September 2024, Pages 269–278
Papa Guedel FAYE1, Samba BALDE2, Claudine Stéphanie Cocote DOS REIS3, Khadim Niane4, Sow Alioune5, Omar Ben Khatab Cissé6, Omar TOURE7, Nicolas Cyrille AYESSOU8, and Mady CISSE9
1 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
2 Laboratoire d’Electrochimie et des Procédés membranaires, Cheikh Anta Diop University, Ecole Supérieure Polytechnique, Dakar, Senegal
3 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
4 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
5 Laboratoire d’Electrochimie et des Procédés Membranaires (LEPM), ESP-UCAD, Dakar, Senegal
6 Iba Der THIAM University, National Higher School of Agriculture, BP A296 Thiès, Senegal
7 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
8 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
9 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
Original language: English
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The objective of this work is to evaluate the content of total polyphenols in the leaves and stem of M. charantia. Three extraction methods (decoction, infusion and maceration) were used for a period of 60 minutes with a sample every 20 minutes. The conductivity and the coloring were evaluated on these different extracts. The total phenolic content of the extracts was estimated by Folin’s method. The color of the samples was measured based on the CIELAB color system using a colorimeter (KONICA MINOLTA-Japan). The results were processed with R software version 3.2.4 Revised (2018-03-16, R-70336) and Minitab 18 software. The results showed that decoction is the best method for extracting total polyphenols with 1.69 gAG.100.g-1 of extract, followed by infusion and maceration respectively 0.61 gAG.100.g-1 and 0.32 gAG.100.g-1. The analysis of the color of the extracts reveals that the intensity of the yellow color is more accentuated in decoction. The comparison of the content of phenolic compounds in the leaves and stems showed that the leaves and stems of M. charantia from the Fatick region are richer in total polyphenols than of M. charantia from the Dakar region.
Author Keywords: decoction, infusion, maceration, polyphenols, extraction, M. charantia.
Papa Guedel FAYE1, Samba BALDE2, Claudine Stéphanie Cocote DOS REIS3, Khadim Niane4, Sow Alioune5, Omar Ben Khatab Cissé6, Omar TOURE7, Nicolas Cyrille AYESSOU8, and Mady CISSE9
1 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
2 Laboratoire d’Electrochimie et des Procédés membranaires, Cheikh Anta Diop University, Ecole Supérieure Polytechnique, Dakar, Senegal
3 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
4 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
5 Laboratoire d’Electrochimie et des Procédés Membranaires (LEPM), ESP-UCAD, Dakar, Senegal
6 Iba Der THIAM University, National Higher School of Agriculture, BP A296 Thiès, Senegal
7 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
8 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
9 Cheikh Anta Diop University, Ecole Supérieure Polytechnique de Dakar, Department of Chemical Engineering and Applied Biology, Water, Energy, Environment and Industrial Processes Laboratory (LE3PI), BP 5080 Dakar Fann, Senegal
Original language: English
Copyright © 2024 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The objective of this work is to evaluate the content of total polyphenols in the leaves and stem of M. charantia. Three extraction methods (decoction, infusion and maceration) were used for a period of 60 minutes with a sample every 20 minutes. The conductivity and the coloring were evaluated on these different extracts. The total phenolic content of the extracts was estimated by Folin’s method. The color of the samples was measured based on the CIELAB color system using a colorimeter (KONICA MINOLTA-Japan). The results were processed with R software version 3.2.4 Revised (2018-03-16, R-70336) and Minitab 18 software. The results showed that decoction is the best method for extracting total polyphenols with 1.69 gAG.100.g-1 of extract, followed by infusion and maceration respectively 0.61 gAG.100.g-1 and 0.32 gAG.100.g-1. The analysis of the color of the extracts reveals that the intensity of the yellow color is more accentuated in decoction. The comparison of the content of phenolic compounds in the leaves and stems showed that the leaves and stems of M. charantia from the Fatick region are richer in total polyphenols than of M. charantia from the Dakar region.
Author Keywords: decoction, infusion, maceration, polyphenols, extraction, M. charantia.
How to Cite this Article
Papa Guedel FAYE, Samba BALDE, Claudine Stéphanie Cocote DOS REIS, Khadim Niane, Sow Alioune, Omar Ben Khatab Cissé, Omar TOURE, Nicolas Cyrille AYESSOU, and Mady CISSE, “Extraction and comparison of phenolic compounds obtained using three different methods on Momordica leaves and stems charantia linn,” International Journal of Innovation and Scientific Research, vol. 74, no. 2, pp. 269–278, September 2024.
