Breastfeeding is essential in infant’s growth and development. Thus, several thousand children suffer from insufficient breastfeeding. Dealing with that, Tigernut (cyperus esculentus L.) is used by rural populations for his Lactogenic properties. This study aims to bring out the potential of nut on mammary gland development and milk production in wistar mice by the histological approach. Mice are divided into four groups; groups 1 and 2 represent respectively the negative control group (rabbit food and water) and the positive control (rabbit food and medicine standard), groups 3 and 4 are tests groups and are fed by tigernut. The histological sections of the different groups were observed using an photonic microscope OPTIKA 4083 B3. A significant development of the mammary glands is noted in the mice of lots 3 and 4, an expansion of the alveoli, and glandular ducts was observed, the connective tissue is invaded by many dense fat cells, the muscle bundles are denser and held the milk ducts are filled with dense granules of protein aceous nature. In group1 rare fat cells are identified and muscle bundles distended. The mammary glandular units present an empty light, a thick and hypertrophied epithelium with tall (non-active) cylindrical cells. The milk ducts are dilated and empty. Lot 4 (positive control) has clusters of granulations of protein nature, the fat cells are very dense comparable to those of batch 4 and 3. This results confirms that tigernut has lactogenic properties.