Hospital worn water of the University Private clinics of Kinshasa (Democratic Republic of Congo) was evaluated for ecotoxicological tests. The physicochemical parameters of the effluents of the university private clinics of Kinshasa taken in situ and ex situ test a variation active either with the rise for some, or with the fall for others. On the ecotoxicological level, worn water of the university private clinics has a toxicity against the species halieutics such is the concrete cases Gambusia affinis which were useful to us like indicator of toxicity of these effluents .On the four sites of taking away, it was noted that the rate of survivors decreased of a site with another. The rough concentrations (100ml) of the effluents did not grant less chance to the individuals to survive. The effluents are toxic with a lethal concentration going from 52ml to 100ml. After a test of treatment in UASB of the whole of the effluents of the university private clinics obtained at the various outlets, the effluent has a very low toxicity at the populations of Gambusia affinis. After treatment, this effluent is toxic with a lethal concentration around 100ml.