The objective of this paper is generation of an algorithm that will separate moving foreground from a stationary background in a general video sequence. We use the different models to calculate the foreground motion in a robust estimation framework. Segmentation of objects in image sequence is very important in many aspects of multimedia applications. We describe a system for representing moving images from the multi-layered sequence. This work realizes a motion- based image isolation algorithms for isolating the moving image in a multi-layered moving image sequence. The system has been proposed here which can efficiently segment a moving foreground object from a given image sequence with still background. The modules of the system are developed using MATLAB and verified for its functionality. In our system different algorithms like LMSE, Block-matching algorithm, motion tracing and recursive algorithms are used to estimate foreground image segmentation for Multi-layered video sequence. Experimental results are given to show the efficiency of our methods.