According to the World Health Organization, more than 80% of the population in Africa resort to traditional medicine to solve the primary problem of health. In the present study, an ethno-botanical survey was carried out in six markets of the district of Lukunga according to principles' included in the declaration of Helsinki. The ethno-botanical data collected were then supplemented by information concerning the plant ecological types. The results from this study revealed that 39 species belonging 37 genera and 25 families were inventoried in the medicinal flora of the District of Lukunga in Kinshasa city. These plant species treat 31 diseases; however the best consensus emerges between inquired only for the treatment of malaria and diabetes mellitus (ICF ≥ 50%). While according to the value of use agreement, only two plant species: Annona senegalensis and Quassia africana displayed interesting result (VAUs ≥ 0.15). Our study revealed that the use of the medicinal plant species is a current practice among the age ranging from 22 to 67 years with prevalence at the old people of 43-49 years. The majority (66%) of the medicinal plants users have a level of secondary studies. The maceration (32%) is the most use mode of preparation while the root is the most used plant part. According to ecological studies, the phanerophytes as well as the Guinean species are prevalent in the medicinal flora of the District of Lukunga. Lastly, the chemical screening revealed the presence in the two selected plant species of the secondary metabolites of biopharmaceutical relevance such as total polyphenols, flavonoids and saponins. It is thus desirable that advanced phytochemical and pharmacological studies are carried out on the two plant species for the scientific validation of their bioactivity (conversion of the traditional knowledge into scientific culture) on the one hand, but also with an aim of both revalorizing and preserving/using them for sustainable development according to the convention on biological diversity on the other hand.