This study wore on the diversity and distribution of ophidians in a few protected areas in the Oriental Province (DR. Congo). The harvest data were performed at Tshuapa Lomami Lualaba Park (TL2), in the Yoko Forest Reserve (RFY) and in the Rubitele hunting area (RBTL). The collections come from the missions of Tshuapa Lomami Lualaba Park (TL2), the Yoko Forest Reserve (FRY) and Rubitele hunting area (RBTL). The Ophidians specimens were captured in actively prospecting night with a snake cane; some specimens of Ophidians were purchased from villagers. In total 91 biopsies were taken from the specimens examined belonging to 7 families 25 genera and 28 species. Using a unique method of catching sight in the three protected areas we find that the dominant species was the horned viper Bitis nasicornis (12.09%), followed by the species Boaedon olivaceus (10.99%) while Grayia smithii, Lycophidion laterale, Dendroaspis jamesoni, Causus maculatus, Natriciteres olivacea, Dasypeltis fasciata, Thrasops jacksonii, Rhamnophis aethiopissa, Thelotornis kirtlandii, Toxicodryas pulverulenta et Hapsidophrys lineatus were less represented with 1.10% for each of them. The index of Shannon - Weiner (H) show that the Yoko Forest Reserve (RFY) (H = 3,898) present a higher diversity of Ophidians than that other protected areas. The Simpson index was 0.81 for Tshuapa Lomami Lualaba park (TL2), 0.918 for Yoko Forest Reserve (RFY) and 0.907 for the Rubitele hunting area (RBTL). The equitability is 0.97; 0.917 and 0.944 respectively for the Tshuapa Lomami Lualaba Park (TL2) for the Yoko Forest Reserve (RFY) and the Rubitele hunting area (RBTL). The Species richness (RS) is 19 for the Yoko Forest Reserve (RFY), 15 for Rubitele hunting area (RBTL) and 6 for Tshuapa Lomami Lualaba Park (TL2).