Present study was conducted to investigate the antimicrobial potential of Amaranthus viridis ethanolic extracts, against two Gram positive bacterial strains, Staphylococcus aureus and Bacillus subtilis, and four Gram negative bacterial strains via; Proteus vulgaris, Peudomonas picketii, Klebsiella pneumonia and Escherichia coli. Disc diffusion method was used and also by calculating their MIC values. Antibacterial assay indicated that Amaranthus viridis had inhibitory activity against Bacillus subtilis, Escherichia coli, Proteus vulgaris and Peudomonas picketii. Agar tube dilution method was used to evaluate the antifungal activity of Amaranthus viridis. Ethanolic extract was tested against five different strains of fungal species, including;Fusarium solani, Alternaria species, Aspergillus flavus green, Aspergillus nigar and Aspergillus fumigatus. It was concluded that Amaranthus viridis had moderate antifungal activity (41-51%) against Alternaria species while low activity (below 40%) against Aspergillus flavus green, Aspergillus nigar and Aspergillus fumigatus. The study revealed antibacterial and antifungal potential of etahnolic extract of Amaranthus viridis.