Introduction: Vitex negundo L. (Nisinda locally), belongs to the family Verbenaceae, found almost everywhere in Bangladesh is a medicinal aromatic shrub. Materials and Methods: An attempt was taken to its micropropagation from field-grown explants (shoot-tip) in Murashige and Skoog medium fortified with various concentrations of phytohormones. Results: Experimentally, the best shoot induction was observed in full strength MS medium supplemented with BAP 1.0mg/L and Kin 0.5mg/L. However, 0.5mg/L IBA in half strength MS medium was enabled to induce 80% root initiation with the highest root number and longest shoot length. Well-developed roots were successfully subjected to hardening process and acclimatized. Conclusion: Regenerated plantlets were same as the natural plants and showed 80.56% survival frequency with frisky and seductive appearances without any abnormalities.