It is estimated that around 3.5 billion people worldwide are affected by toxoplasmosis, the prevalence of which depends on geographical, climatic and socioeconomic conditions. This study was conducted to determine de sero-prevalence of toxoplasmosis among pregnant women attending the antenatal clinic in Bunia Health District. Cross sectional method supported by questionnaire was used in this study. The sample was made up of 375 pregnant women, through multi stage sampling. Content analysis supported by percentage calculation served to analyse data. After analysis, this study revealed that sero-prevalence of toxoplasmosis among pregnant women attending antenatal clinic in Bunia health District is 5.07%. Women aged 15-19 years were the most affected by toxoplasmosis in Bunia Health District, the pregnancy age was of 3-5 months, respectively to primiparous and multiparous. From these results, there is a strong need to strengthen pregnant women awareness on preventative means of toxoplasmosis using contextual strategies in Bunia Health District.