Poitevin marsh is a typical example of anthropogenic activities influence on wetlands. One of the problems to be solved before considering preservation solutions, concerns the improvement of hydrogeological knowledge and water transfers between the marsh and Jurassic carbonate bedrock. This study aims to determine the hydrodynamic parameters of Quaternary and Upper Oxfordian aquifers in order to appreciate the water transfers between these aquifers and to better understand the hydrogeology of this area. Thus, pumping tests are carried out to determine the hydrodynamic parameters of the aquifers. The pumping tests interpretation carried out, allowed to highlight very low permeability of upper Oxfordian formations under Quaternary cover. As for the formations of Quaternary aquifer, obtained permeability coefficients are in the order of 10-7m/s to 10-6m/s with transmissivity values in the order of 10-6 to 10-5 m2/s and a storage coefficient in the order of 10-2. However, Quaternary formations are more permeable than Upper Oxfordian formations. On the time scale of the measurements, no hydraulic connection between Quaternary and Upper Oxfordian aquifers could be observed. The water transfers between these two aquifers would therefore be very limited.