Motivation is considered the heart of the learning process that is affected by many factors, among which there is gender. Therefore, the researcher conducted a quantitative research method to investigate the relationship between gender and motivation sub-themes while using problem-based learning. Nine female students and eleven male students of the second baccalaureate level participated in the current research through using the convenience sampling technique. The quasi-experimental research design, a quantitative research method, is used to conduct this study to analyze cause-effect relationships between the variables being studied. Then, the participants were given a five-point Likert scale questionnaire to fill in. The SPSS software was used to analyze data. The findings demonstrated that there was a positive relationship between gender and students’ achievement and motivation sub-themes.