The aim of this research is to control the quality of Ciprofloxacin used alongside and in the health centers and sold in Niamey city by using Thin Layer Chromatography method to compare the results achieved in order to contribute to informed decision-making for possible interventions. This research aimed to investigate on eleven samples distributed as follows which five samples are from pharmacies, five samples are from street vendors and one sample specialty used as reference to check the quality control of them. The different reagents used are: Methanol, Dichloromethane, Ammonia at 260gL-1 or Pure ammonia, Acetonitrile and 0.1 M NaOH. The migration of all the samples in proposed diluent shows that all of them contain the active ingredient of ciprofloxacin. The percentage of the active ingredient were calculated as per protocol of Clarke’s analysis of drugs and poisons in chemistry guidelines. It varies from 90.90 to 109.09 for pharmacies and 98.18 to 106.35 for the street vendors.According to the results of the different frontal reports, each sample contains the percentage of active principle recommended by WHO which is 90 to 110 % of active ingredient recommended by WHO.This technique of quality control can be used for practical work or tutorial and laboratories where drug quality control mechanism is not often checked.