Introduction: The objective of the study is to investigate the factors associated with trachoma in the Diourbel Health District (HD) in Senegal in 2019. Methodology: This is a cross-sectional, descriptive, and analytical study conducted in June 2019 among households in the Diourbel HD. It was a two-stage random sample survey. Data were collected during an individual interview after free and informed consent. Logistic regression was used to identify factors associated with trachoma. Results: 3544 individuals aged 1 year to 9 years and 3402 individuals aged 15 years and older, respectively in the active trachoma (AT) and trachomatous trichiasis (TT) components were surveyed. Among the 1-9 year olds, 1973 individuals or 55.7% of the respondents were less than 5 years old. 1864 individuals or 52.6% were female. The prevalence of BP was 2.0% [1.5%-2.5%]. Among those aged 15 years and over, 2250 individuals or 66.1% of the respondents were aged 15-44 years. 2498 individuals or 73.4% of the respondents were female. The prevalence of TT was 1.6% [1.1%-2.0%]. Among 1-year-olds to 9-year-olds, the age range 3 years to 4 years: ORA= 2.66 [1.31-5.41] and latrine outside the home: ORA= 17.73 [4.01-78.36] were significantly associated with BP. In the 15+ age group, the age groups 45-64 years: ORA= 17.79 [6.82- 46.37], 65-74 years: ORA= 22.9 [7.98- 65.69], and 75+ years: ORA= 18.83 [5.9- 60.14] were significantly associated with TT. Conclusion: The Diourbel HD validated the WHO criteria for elimination of BP, but TT still remains a public health problem. The risk factors associated with TT were age and latrine outside the home. The risk factor associated with TT was age. Thus, in order to obtain the certificate of elimination of trachoma in the Diourbel Health District by 2024, the focus must be on effective health promotion on the one hand, and on the other hand, on close coordination and multisectoral action in the health sector and beyond.