The Aghien estate, the site of this study, is part of the dynamic of creating a new peri-urban nature reserve. Following the regression of the surface areas of protected areas by human activities within the Ivorian parks and reserves, the partial Aghien nature reserve was created to allow the survival of this particular ecosystem, essential for the preservation of the peri-urban forest and the conservation of biological diversity. The general objective of this study is to assess the floristic diversity of the Partial Nature Reserve of Aghien. To do this, 36 plots of 625m2 (25 m x 25 m) each were first distributed in six (06) biotopes of the partial nature reserve of Aghien (RNPA). These are rubber plantation (n = 6) palm plantation (n = 6), secondary forest (n = 6), swamp meadow (n = 6), fallow land (n = 6), Bare soil (n = 6). Then botanical inventories based on itinerant and surface survey methods were carried out in each plot. A total of 265 species divided into 174 genera and 63 families were inventoried in the RNPA. Finally, the assessment of the floristic composition of the vegetation and that of the floristic composition of the vegetation were determined by the floristic variability and the diversity indices.