[ Effet de différents éléments fertilisants sur le développement de deux espèces de Trichoderma spp. ]
Volume 32, Issue 1, August 2017, Pages 18–29
Hanane El Kaissoumi1, Abdellatif OUAZZANI CHAHDI2, Karima Selmaoui3, Rachid Benkirane4, Amina Ouazzani Touhami5, and Allal Douira6
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
6 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The compatibility of two species of Trichoderma spp. was tested in vitro and in soil with different fertilizing elements (N, P, K, Ca) added to increase concentrations in the solid potato-based medium. After 48 h of incubation, CaCl2, KNO3 and KH2PO4 at 400 mg/L showed an inhibitory effect on mycelial growth of Trichoderma harzianum strain (Tcomp) and Trichoderma viride strain (TV1) compared to controls varying respectively from 9.66 to 28.33 and 10.33 to 29.16 / 90.00 mm. For cons, the NH4NO3, (NH4) 2SO4 and CaSO4, 2H2O had a mean toxicity against the two strains tested, mycelial growth was ranged from 50.50 to 64.00 / 90 mm. After 7 days of incubation, both strains were able to produce conidia; their numbers have varied respectively from 6369.00 to 117833.20 / 245843.00 conidia / mm2 and 6793.00 to 67221.70 / 649635.00 conidia / mm2. The pH was slightly basic in the potato liquid medium before culturing and after the addition of the products at different concentrations (7.64-8.60). The pH became acid after culturing of the two strains; it varied between 3.13 and 5.85. Fresh weights were low in the presence of 50 mg / L of Calcium Chloride and medium for other products from 0.01 to 2.79 and 3.04 to 6.69 respectively compared to control 7.31 - 9.28 mg. Dry weights were low in the presence of the tested products compared to controls from 7.31 - 9.28 mg. In soil, both strains were able to grow on barley straw fragments at a concentration of 400 mg/L. The colonization percentage varied respectively between 63% and 94%. Two strains of Trichoderma showed substantial compatibility with the most part of fertilizers tested in vitro and a great capacity to keep the soil in the presence of different elements. Thus, the antipollution properties of Trichoderma spp. can be used in agriculture in soils with excess of nitrogen and phosphate. They also have the ability to increase the capacity of nutrient uptake by plants.
Author Keywords: Trichoderma harzianum, Trichoderma viride, fertilizing elements, mycelial growth, conidia production, weight, saprotrophytic activity.
Volume 32, Issue 1, August 2017, Pages 18–29
Hanane El Kaissoumi1, Abdellatif OUAZZANI CHAHDI2, Karima Selmaoui3, Rachid Benkirane4, Amina Ouazzani Touhami5, and Allal Douira6
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
6 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2017 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The compatibility of two species of Trichoderma spp. was tested in vitro and in soil with different fertilizing elements (N, P, K, Ca) added to increase concentrations in the solid potato-based medium. After 48 h of incubation, CaCl2, KNO3 and KH2PO4 at 400 mg/L showed an inhibitory effect on mycelial growth of Trichoderma harzianum strain (Tcomp) and Trichoderma viride strain (TV1) compared to controls varying respectively from 9.66 to 28.33 and 10.33 to 29.16 / 90.00 mm. For cons, the NH4NO3, (NH4) 2SO4 and CaSO4, 2H2O had a mean toxicity against the two strains tested, mycelial growth was ranged from 50.50 to 64.00 / 90 mm. After 7 days of incubation, both strains were able to produce conidia; their numbers have varied respectively from 6369.00 to 117833.20 / 245843.00 conidia / mm2 and 6793.00 to 67221.70 / 649635.00 conidia / mm2. The pH was slightly basic in the potato liquid medium before culturing and after the addition of the products at different concentrations (7.64-8.60). The pH became acid after culturing of the two strains; it varied between 3.13 and 5.85. Fresh weights were low in the presence of 50 mg / L of Calcium Chloride and medium for other products from 0.01 to 2.79 and 3.04 to 6.69 respectively compared to control 7.31 - 9.28 mg. Dry weights were low in the presence of the tested products compared to controls from 7.31 - 9.28 mg. In soil, both strains were able to grow on barley straw fragments at a concentration of 400 mg/L. The colonization percentage varied respectively between 63% and 94%. Two strains of Trichoderma showed substantial compatibility with the most part of fertilizers tested in vitro and a great capacity to keep the soil in the presence of different elements. Thus, the antipollution properties of Trichoderma spp. can be used in agriculture in soils with excess of nitrogen and phosphate. They also have the ability to increase the capacity of nutrient uptake by plants.
Author Keywords: Trichoderma harzianum, Trichoderma viride, fertilizing elements, mycelial growth, conidia production, weight, saprotrophytic activity.
Abstract: (french)
La compatibilité de deux espèces de Trichoderma spp. est testée in vitro et dans le sol avec différents éléments fertilisants (N, P, K, Ca) additionnés à des concentrations croissantes dans le milieu à base de pomme de terre solide. Après 48 h d’incubation, le CaCl2, le KNO3 et le KH2PO4 à la concentration de 400 mg/L ont montré un effet inhibiteur sur la croissance mycélienne de la souche Tcomp de Trichoderma harzianum et TV1 de Trichoderma viride par rapport aux témoins variant respectivement de 9,66 à 28,33 et 10,33 à 29,16/90,00 mm. Par contre, le NH4NO3, le (NH4)2SO4 et le CaSO4, 2H2O ont présenté une toxicité moyenne vis-à-vis des deux souches testées, la croissance mycélienne a varié de 50,50 à 64,00/90 mm. Après 7 jours d´incubation, les deux souches ont été capables de produire des conidies, leurs nombres ont varié respectivement de 6369,00 à 117833,20/245843,00 conidies/mm2 et 6793,00 à 67221,70/649635,00 conidies/mm2. Le pH du milieu liquide à base de pomme de terre est légèrement basique avant la mise en culture et après l’addition des produits à différentes concentrations (7,64-8,60). Il est devenu acide après la culture des deux souches de Trichoderma, il a varié entre 3,13 et 5,82. Les poids frais ont été faibles en présence de 50 mg/L du Chlorure de Calcium et moyens pour les autres produits respectivement 0,01-2,79 et 3,04-6,69 par rapport aux témoins 7,31-9,28 mg. Les poids secs ont été faibles en présence des produits testés par rapport aux témoins 7,31-9,28 mg. Dans le sol, les deux souches ont été capables de se maintenir sur les fragments de paille d´orge à une concentration de 400 mg/L. Le pourcentage de colonisation a varié de 63% à 94%. Les deux souches de Trichoderma ont montré une compatibilité importante avec la plus part des fertilisants testés in vitro et une grande capacité de se maintenir dans le sol en présence de différents éléments. Ainsi, les propriétés dépolluantes des Trichoderma spp. pourront être utilisées en agriculture dans les sols présentant un excès d’azote et de phosphate. Ils ont aussi la capacité d’augmenter la capacité d’absorption des éléments fertilisants par les plantes.
Author Keywords: Trichoderma harzianum, Trichoderma viride, éléments fertilisants, croissance mycélienne, production des conidies, poids, activité saprophytique.
How to Cite this Article
Hanane El Kaissoumi, Abdellatif OUAZZANI CHAHDI, Karima Selmaoui, Rachid Benkirane, Amina Ouazzani Touhami, and Allal Douira, “Effect of different fertilizing elements on the development of two species of Trichoderma spp.,” International Journal of Innovation and Scientific Research, vol. 32, no. 1, pp. 18–29, August 2017.
