Volume 12, Issue 2, December 2014, Pages 480–483
Shamima Nasrin1, Md. Shamim Hossain2, Md. Ashrafuzzaman Sapon3, and Monokesh Kumer Sen4
1 Scientific Officer, Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka, Bangladesh
2 Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
3 Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
4 Bioscience Division, Graduate school of Science & Technology, Shizuoka University, Shizuoka, Japan
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Introduction: Vitex negundo L. (Nisinda locally), belongs to the family Verbenaceae, found almost everywhere in Bangladesh is a medicinal aromatic shrub. Materials and Methods: An attempt was taken to its micropropagation from field-grown explants (shoot-tip) in Murashige and Skoog medium fortified with various concentrations of phytohormones. Results: Experimentally, the best shoot induction was observed in full strength MS medium supplemented with BAP 1.0mg/L and Kin 0.5mg/L. However, 0.5mg/L IBA in half strength MS medium was enabled to induce 80% root initiation with the highest root number and longest shoot length. Well-developed roots were successfully subjected to hardening process and acclimatized. Conclusion: Regenerated plantlets were same as the natural plants and showed 80.56% survival frequency with frisky and seductive appearances without any abnormalities.
Author Keywords: Vitex negundo, micropropagation, Murashige and Skoog medium, acclimatized.
Shamima Nasrin1, Md. Shamim Hossain2, Md. Ashrafuzzaman Sapon3, and Monokesh Kumer Sen4
1 Scientific Officer, Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka, Bangladesh
2 Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
3 Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
4 Bioscience Division, Graduate school of Science & Technology, Shizuoka University, Shizuoka, Japan
Original language: English
Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Introduction: Vitex negundo L. (Nisinda locally), belongs to the family Verbenaceae, found almost everywhere in Bangladesh is a medicinal aromatic shrub. Materials and Methods: An attempt was taken to its micropropagation from field-grown explants (shoot-tip) in Murashige and Skoog medium fortified with various concentrations of phytohormones. Results: Experimentally, the best shoot induction was observed in full strength MS medium supplemented with BAP 1.0mg/L and Kin 0.5mg/L. However, 0.5mg/L IBA in half strength MS medium was enabled to induce 80% root initiation with the highest root number and longest shoot length. Well-developed roots were successfully subjected to hardening process and acclimatized. Conclusion: Regenerated plantlets were same as the natural plants and showed 80.56% survival frequency with frisky and seductive appearances without any abnormalities.
Author Keywords: Vitex negundo, micropropagation, Murashige and Skoog medium, acclimatized.
How to Cite this Article
Shamima Nasrin, Md. Shamim Hossain, Md. Ashrafuzzaman Sapon, and Monokesh Kumer Sen, “Micropropagation of Vitex negundo L. - A Significant Medicinal Plant,” International Journal of Innovation and Scientific Research, vol. 12, no. 2, pp. 480–483, December 2014.
