Carrying on the validation instrument measuring Interactional profile of teachers through the teacher-pupils relation in the classes of sixth years primary of Isiro, this study want propose a tool of measure of the main-pupils relation in our schools. It is while basing us on the Trainee's Profile Interactional (PIS) that we constructed and arranged the structure and the items of the questionnaire. This instrument is composed of 30 items regroup in four measurements. All main tests for a plausible factorial solution used at the time of two analyses proved to be satisfactory. Is our KMO value of 0.80, is the test of sphericity meaningful (Chi square = 5560.15 to p = 0.00< 0.05). Also, the value of α coefficient (0.68) is acceptable. These results indicate the validity of constructs of our questionnaire. Taken into consideration these results, we reached our objective, the one to construct a psychometric instrument from our model of the departure to measure the quality of the Teacher-pupils relation in the classes of sixth year’s primary of Isiro. The final structure of this instrument (Interactional Profile of the Teacher) is constituted of four factors and 30 items.