The objective of this study was to evaluate the susceptibility of malaria vectors to pyrethroids and to detect the presence of kdr mutations in two peripheral agricultural sites in the city of Mouila. The study was conducted during the rainy season in 2017. Anopheles were collected at the larval stage by the "dipping" method and reared in the laboratory until the adult stage. Susceptibility tests were performed on the F0 generations according to the protocol recommended by WHO. The insecticides used were alphacypermethrin (0.05%, 0.25%, 0.50%), cyfluthrin (0.15%, 0.75%), etofenprox (0.5%, 5%) and bifenthrin 0.20%. The individuals of Anopheles gambiae s.l. from the tests were identified by the PCR technique. The survivor and dead mosquitoes were used to detect kdr mutations via PCR. The members of the An. gambiae complex of the study sites were composed of two species, An. gambiae s.s and An. coluzzii, with a predominance of An. gambiae s.s. The test results showed a resistance to alphacypermethrin (0.05%, 0.25% and 0.50%), cyfluthrin 0.15%, etofenprox 0.5% and bifenthrin 0.20% in both study sites and a suspicion of resistance to etofenprox 2.5% in the Moutassou site. Populations of An. gambiae s.l were susceptible to cyfluthrin 0.75% in these study sites (≤ 98%) and etofenprox 2.5% in the Mboukou site (100%). These two insecticides could be used for vector control after resistance has been observed to permethrin and deltamethrin (at low doses).