This study proposes a conceptual model that investigates the factors affecting nurses' behavioral intention to use a Blended E-Learning System (BELS). We integrated motivation, the Theory of Planned Behavior (TPB), and the Technology Acceptance Model (TAM) to create a theoretical model to explain and predict the intentions of learners to use BELS. Additionally, in the research model, motivation is proposed as a formative second-order construct driven by intrinsic motivation. Self-reporting questionnaires were distributed to local community hospitals, regional hospitals, and medical centers in Taiwan. Among the 589 distributed questionnaires, we received 528 valid responses, yielding a response rate of 89.64%. Using structural equation modeling (SEM), the results indicated that perceived usefulness, attitude, and subjective norms influence behavioral intentions toward BELS. Perceived ease of use, perceived usefulness, and motivation considerably affect attitudes related to use. The present study demonstrated that the two antecedents, intrinsic and extrinsic of motivation, are crucial components of motivation. This study concludes with a discussion on the limitations of the study, and its implications in further research and practice.