Coconut fiber is the substrate of choice for the production of plants in vegetable nursery above ground Gabon. However, procurement and production costs of the material remain high. This study conducted in the experimental area of the company Horta Gabon, located in the town of Akanda north of Libreville in Gabon, with the aims to test the possibility of substituting coconut fiber imported by coffee husks produced locally. Thus, through monitoring of the germination and vegetative behavior of Tomato seedlings (Lycopersicon esculentum) subject to various disinfection treatments, which have focused on the nature of disinfectant and the volumes applied, have showed that plants of tomato products on coffee husks disinfected 1.5 L of Metam Sodium (MS) and washed have better returns in terms of germination and development of tomato seedlings. These results indicate that the coffee husk could be an alternative substrate of coconut fiber without causing major imbalances.