Durum wheat is an important crop in North Africa and Morocco. Hessian fly is the most important insect pest of wheat in the dryland areas in Morocco. Breeding wheat for genetic resistance to pests is the most sustainable strategy. The objective of this study is to assess the genetic diversity of quality criteria in a recombinant population segregated for resistance to Hessian fly and to verify the effects of the presence of H.fly resistance genes on these criteria. Grains of 180 recombinant inbred lines derived from hybrid (Cando-cross-H25 / CI115 // CM829 / BZAIZ-AHF) were analyzed for composition of high molecular weight-glutenin subunits and low molecular weight-glutenin subunits (HMW-GS and LMW-GS). The reaction of these lines to Hessian fly being previously known, these results were compared between H. fly resistant and susceptible lines. Electrophoretic analysis revealed six different bands. The various combinations of these bands gave eight allelic forms encoded by Glu B1 locus. Two allelic classes were observed for low molecular weight-glutenin subunits, they are LMW-SG-1 and LWM-SG-2. The protein composition of this collection presents a great variability that will be useful to breeders by allowing choosing the useful alleles and broadening the genetic base. The characteristics studied were found similar between classes of H. fly resistant and susceptible lines.