For the past decades, many banks and financial institutions around the world have been motivated in implementing electronic banking due to the fast advances in information technology. Despite the significant growth of informatics in banking, electronic banking is still underutilized among banks and other financial institution in Ghana. This study, therefore, enriches the current knowledge on influential factors that affect individual behavioral intention on adopting e-banking. Thus, this study employs Technology Acceptance Model (TAM) in examining the factors that affect person’s behavioral intention in adopting e-banking by expanding it with three other variables which are prior internet knowledge, Information on e-banking and perceived credibility. Survey questionnaire was randomly distributed to a total of 210 respondents who are customers of public and private banks located in Kumasi, Ghana. In all 193 were fully completed representing of 91 percentage. On average, the results indicates that perceived usefulness, perceived ease of use, prior Internet Knowledge, Information on Electronic Banking and Perceived Credibility are significant determinants of customers intention in E-Banking using TAM. This outcome reflects both theoretical and practical consideration in promoting e-banking services in the current competitive industry in Ghana.