The persistence of Escherichia coli and Salmonella typhi in streams around epidemic environment was investigated. Water samples were collected from 15 streams viz; Abe koko, Abe mango Adele, Afo, Asin, Bank road, Fajuyi, Falegan, Fiyinfolu, Housing estate, Ilawe road, Odo Ado, Odo arin, Odofumi and Omisanjana; all in Ado- Ekiti. From the water sample tested Adele and Ilawe road were found to have the highest microbial load of 112x103cfu/mL and 104x103cfu/ml respectively while Odo Arin was found to have the lowest microbial load of 34x103cfu/mL. Biochemical teste were also performed in order to certify the organism isolated. These organisms are Escherichia coli and Salmonella spp. The streams examined in Ado-Ekiti metropolis had unsatisfactory levels of contamination with E.coli, and other pathogenic microorganisms which render the water from the streams unsuitable for human use. The results show that the occupants of these environments are unhygienic and disposal activities are below standard.