Background: The endemic of toxoplasmosis infection is considered high in Yemen, but there has been inadequate information on the prevalence rate and risk determinants among female at age bearing time in Yemen. This study shed some light on the epidemiology of Toxoplasmosis among women in Yemen. The aim of the present study to determine the Seroprevalence of T. gondii antibodies among pregnant and non pregnant women in Sana'a city, its associated risks exposures as age, residence, education, history of abortion, contact with animals and undercooked meat or food. Method: This study was a cross-sectional study, the size of sample was 200, after a questionnaire applied to the pregnant and none pregnant women, anti-Toxoplasma IgG, IgM antibodies were studied by (ECL). Results: 86 samples 43% were seropositive for anti-Toxoplasma gondii IgG and six samples 3% were seropositive for anti-Toxoplasma gondii IgM. In The positive samples (86) for IgG the mean age of the women was ≥ 15 years and, the rate was 5% for age group 15-19 years, 16% for 20-24 years, 22%for 25-29 years, 20% for 30-34years, (20%) for35-39% and 17% for ≥ 40 years and, the prevalence rate of IgG 48.3% for females who, had abortion and 38.9% for females who hadn't abortion. For the residency 66% of infection was in urban, 34% was in rural. Conclusion: Increased Seroprevalence of Toxoplasmosis.