Studies on antifungal activity of honey with the objectives of determining the susceptibility of fungal isolate at different dilution of honey was carried out, medically important samples of fungi were collected and isolated from hospital and used in the bioassay following a modified Kirby-bauer technique. The aim of the research was to determine the current level of effectiveness of honey on some humans and animal dermatophyte. The honey was collected from its hunters and vendors in Kano state northern part of Nigeria. Result showed that the higher the concentration of honey the higher the zone diameter of inhibition. Surprisingly, the zones diameter of inhibition at dilution of 20%v/v and 30% v/v induce and increase susceptibility of fungal isolate tested. The finding showed that honey can be used to treat skin infection associated with Trichophyton tonsurans, Microsporum canis and Aspergillus species through topical application.