Use of doubled haploïdes allows to shorten considerably the cycle of classic selection and to obtain quickly a stable homozygotie. However, regeneration of albino plants, particularly for cereal, constitutes the major problem of this approach. Obtaining haplodiploïdes by in vitro culture of not fertilized ovaries is determined by numerous factors biological as physical or chemical. Our work concerned the optimization of the answer gynogenetique of three varieties of durum wheat (Cocorit, Isly, Jori) cultivated on two different media (M1 et M2) and subjected to a cold pre-treatment at 4 ° C for 7, 11 and 15 days.This work has demonstrated that genotypes interact strongly with pre-treatment duration and with induction medium composition. Thus, a beneficial effect of thermal pre-treatment was noted on the induction of embryos and production of callus, and also on the quality of regenerating. For the regeneration of green plants, the response of genotypes varied with the induction medium composition and with cold pre-treatment period. Indeed, M1 medium was favourable for Cocorit and Jori varieties but with different incubation duration. For cons, M2 medium gave better results with Isly genotype for 7 days incubation.