The present study was carried out to detect the effect of two Auxin namely dichlorophenoxy acetic acid (2, 4-D) and naphthalene acetic acid (NAA) on callus induction from cotyledon and hypocotyledon explants of cassia senna sp. Two explant from in vitro cassia micro plant were used for Callus induction on Murashige and Skoog media (MS) media, as basal media (control) or supplemented with different concentration of 2, 4, - D dichlorophenoxy acetic acid (2,4,D) and naphthalene acetic acid (NAA). On MS basal media (without growth regulators) no callusing obtained. Cotyledon and hypocotyls explants obtained callusing on MS media supplemented with NAA and 2, 4-D at all concentration . Callus growth percentage of cotyledonary leaf segments is varied from 60% to 100%, while the callus growth percentage of hypocotyl segment explant is varied from 75%-to 100%. Highest percentage of callus (100%) was observed in medium supplemented with 2.5 mg/l of the both NAA and 2.4-D. The both explant induce compact callus with all treatment .For callus color , cotyledon explant produced yellowish callus with the both auxin , on the other hand hypocotyls explant produced green to light green callus The highest callus degree (2.55±0.11) was obtained in MS media supplemented with 2.5 mg/l NAA by cotyledon explant , followed by (2.45± 0.15) induced in MS media fortified with 2.5 mg/l NAA(the same treatment) from hypocotyl explant, during same period of time(14 day).