This study aims to quantify the pollution levels in the main Lakes of El Kala National Park; Mellah, Oubeira, and Tonga, which constitute an important source of water supply and a National Heritage according to Ramsar convention, by monitoring five heavy metals (Al, Cr, Zn, Cu, Pb) and the phosphates content. This park contains up to 33% of the population of El Kala district, which liquid wastes are discharged directly in the lakes ecosystem. Results revealed that the Aluminum is present in the three Lakes, Lead was detected in the lakes of Oubeira and Mellah, Zinc and Cr were inexistent in the three lakes, Copper was detected only in Oubeira Lake. Phosphates were present in three lakes with a maximal content of 6 mg/l. This value remains superior to the limit content of phosphates for a lake eutrophication (0.2 mg/l). This situation threatens seriously the Lakes water quality and then its ecosystem.