Department of Molecular Biology and Biotechnology, Pan African University Institute of Basic Science, Technology and Innovation at Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya
Aims: This study focused on the assessment and identification of key insect pests on sweet oranges (Citrus sinensis) plantation in Tony farm of Dire Dawa town. It was also explain the abundance of key pests and effective controlling methods. Methodology and Results: The farm lands of sweet oranges stratified in to nursery stage and adult stage by using stratification method technique. Then random sampling method of data collection was carried out in both strata. By this method direct observations were taken place and the data collected from this area were analyzed. The larvae and adult stages of key insect pests of sweet oranges were identified by using the dissecting microscope. Citrus leaf miners, leaf dogs and cottony cushion scale are key insect pests of sweet oranges in this area. From these citrus leaf miners are highly dominant where as orange dog is less in abundance. Conclusions: Citrus sinensis plants in Tony farm are highly damaged by these key insect pests particularly nursery stages of plants are devastated.
Aims: Pollution of the environment with toxic heavy metals is spreading throughout the world with industrial progress. Metal pollution in industrial areas is of serious environmental concern as these metals like chromium (Cr), nickel (Ni), mercury (Hg), cadmium (Cd), lead (Pb) and cupper (Cu) are known to cause damage to living organisms including human beings. The study investigated the level of heavy metals and antibiotics resistance in bacteria isolated from rhizosphere soils contaminated with tannery effluents. Methodology and Results: Soil samples were collected from three selected rhizosphere soils of the Tannery effluent contaminated environments and heavy metals resistant rhizobacteria were isolated from soil. A total of twenty one rhizobacteria were isolated from Potassium dichromate supplemented nutrient agar. These isolates were categorized under Pseudomonas species, Staphylococcus, Clostridium, Klebsiella species, Bacillus species, Listeria species, and Streptococcus species after biochemical tests. Further, these isolates were assessed for resistance to other heavy metals and antibiotic resistance. Most of these isolate exhibiting maximum resistance against both metals and antibiotics. Conclusion, Significance and Impact of study: These heavy metal resistant bacteria can be useful for the bioremediation of heavy metal contaminated environment including industrial effluents.