The Most of Hospitals are making a surgical treatments having a lot of medical waste it contains large volumes of water in their surgical operations .But during this process the water gets contaminated. This implies the need for waste water treatment before discharge. Waste treatment refers to the activities required to ensure that waste has the least practicable impact on the environment. Improvement in determining the effects of medical waste discharges have led to the passage of stringent environmental laws, which defined the degree of treatment necessary to protect water quality. In this present study enhanced the reactor designed and different parameter. The anaerobic treatment is considered one of the most efficient methods for treating several types of effluents. This is due to its scope for treating high rate of simply biodegradable matters and wastewater. The up flow anaerobic sludge blanket (UASB) technology was considered as the most popular method in which the highest rate of organic materials can be removed. Nonetheless, the long start up interval of UASB reactor requires more understanding of the biological process inside the reactor. During this study, which lasted for 50 days, two distinct phases were carried out according to the ambient temperature. The temperature of the waste water entering the reactor was naturally ranged from 260c to 30o c and no heat exchanger was used. From this experimental work enhanced for treatment efficiency of effluent by varying the different parameters like hydraulic retention time and various organic loading rates. On the basis of the results in optimal hydraulic retention time and organic loading rate BOD, COD and TSS was removed respectively.