A number of studies evidenced the psychological problems being faced by university students. However, little work has done in Pakistan with respect to psychological problems faced by university students and its impact on their self-esteem. On the basis of literature review it was hypothesized that 1) There would be a significant inverse relationship between symptoms of psychopathology and self-esteem. 2) There will be a difference between symptoms of psychopathology among men and women university students. Sample of 400 participants had been taken from major representative departments of Humanities, Sciences, and Social Sciences, Management and administrative sciences of University of Karachi, while using Systematic Random Sampling. Consent form was got signed from every participant. Interview sheet and General Health Questionnaire: GHQ-28 and Rosenberg Self-Esteem Scale (RSES) was administered on each participant. Result analysis revealed that a correlation between symptoms of psychopathology and self-esteem is negatively associated and significant at 0.01alpha level. However, no gender difference found in the symptoms of psychopathology.