Cytomegalovirus is one of the opportunistic infections associated with significantly high morbidity and mortality among patients living with immunodeficiency syndrome. Cytomegaloviruses (CMV) are deoxyribonucleic acid (DNA) viruses and are members of the Herpesviridae family CMV are recognized pathogens that cause community-acquired pneumonia (CAP) in compromised hosts. We investigated the prevalence of CMV among seropositive cases of human immunodeficiency virus (HIV) from Northern India. In this study we observed the relationship between CMV infections among the immunocompromised patients. This study carried out in Department of Microbiology, King George's Medical University (KGMU), Lucknow and Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, Uttarakhand, India. Total 250 HIV sero-positive cases were considered in study of CMV infection. HIV status was confirmed by Enzyme linked immunosorbent assay (ELISA) done at antiretroviral (ART) center, KGMU, Lucknow. CMV positivity was evaluated by CMV- IgG and IgM antibodies using ELISA kit. Among 250 HIV positive cases 10.4% were found positive for CMV- IgG where as 8.4% were positive for CMV- IgM however 1.2% were found to be positive for both CMV- IgG and CMV- IgM. Sero-prevalence of CMV IgG and IgM among HIV infected cases were 10.4% and 8.0% respectively. No significant difference was observed among gender and age group in CMV infection among HIV-positive cases.