Thirty high activity yeast strains saccharolytic and acidifying were isolated from sugar cane press juice and molasses from a sugar plant (grown on a solid semi-synthetic culture medium supplemented with 3 g of sucrose and incubated for 72 h at 40 ° C. the purification of the strains is verified by microscopic control after three subculture cycles in liquid medium and a passage on solid medium in a Petri dish 80 mm in diameter. the identification was carried out according to biochemical tests, physico-chemical and microscopic. These culture, purification and identification have isolated a strain which we named LES16 characterized by its temperature tolerance, which considerably increases during fermentation. This is a Candida sp which presents optimal conditions (pH 5, 3 g / l sucrose, 1 g / l (NH4) 2SO4 and 40 ° C) an enzyme activity of 4228 IU / l. has a major interest in the application of a technique primarily based on the use of a mixed starter containing lactic acid bacteria and yeast fermentation and strong acidifying power. Localization of beta-fructofuranosidase activity LES16 strain was obtained by culture on the semi-synthetic liquid medium supplemented with 3 g of sucrose. The centrifuge technology and cell lysis have produced more cell fractions to search for enzyme activity.