The present study reveals the qualitative and quantitative analysis of the major bioactive constituents of different parts such as root, stem, leaf, flower and latex of medicinally important plant Baliospermum montanum using seven different solvents. Qualitative study of Alkaloids, Carbohydrates, Glycosides, Steroids, Flavonoids, Coumarins, Saponins, Fatty acids, Tannins, Protein and Amino Acids, Gum and Mucilage, Terpenoids, anthroquinones and Phenols showed variable of results in different solvents. Quantitative estimation revealed that phytochemicals are in between the following range alkaloids (6.2 -9.5%), flavonoids (35.63-43.33%), Saponins (9.9 to 13.2%), phenolics (38.43 to 43.44%), tannins (13.26 to 18.3%), terpenoids (25.36 to 33.4%).
In the present investigation, an attempt has been made to accelerate the flavonoid synthesis in Baliospermum montanum (Wild.) Muell-Arg., Dryptes roxburghii (Wall.) Huresawa and Codiaeum variegatum (L.) Bl, members of Euphorbiaceae family. Internodal explants were used to induce callus. Maximum growth of callus was observed in Baliospermum montanum and Codiaeum variegatum at 2mg/L and 3mg/L of 2,4-D respectively whereas Dryptes roxburghii showed maximum growth of callus at 3mg/L of BAP. Suspension culture was obtained from well developed callus. Three elicitors were used to induce flavonoid synthesis namely phenylalanine, copper sulphate and Pseudomonas aeruginosa. Among three, phenylalanine was found to be the best elicitor and maximum production of flavonoid was observed in Baliospermum montanum.
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