Exclusive breastfeeding is the best nourishment for an infant. However, the rate of exclusive breastfeeding remains low to this day. The present work aims to improve the care of women suffering from hypogalactia through the use of pumpkin seeds. Indeed, squash seeds from the Cucurbitaceae family possessing elements characteristic of lactogenic plants are used for the production of milk according to nutritional surveys. To verify this claim, biochemical analyzes concerning proteins, lipids, carbohydrates, ashes, dietary fibers were carried out. Also, the phytochemical parameters were measured. Furthermore, the prolactin level in treated female rats was investigated. The study revealed significant levels of proteins (22.31 ± 0.02 g/100g), lipids (50.56 ± 0.02 g/100g), carbohydrates (9 ± 0.12 g/100g), ash (3.33 ± 0.08 g/100g), dietary fiber (11.04 ± 0.02 g/100g). The results of the phytochemical screening reveal that the polyphenol content is 20.12 mgEQ/gDM) and that of flavonoids is 11.74 mgEAG/gDM. Furthermore, animal experiments revealed a significantly higher prolactin level in female rats having received Citrullus lanatus powder (57.44 ng/ml) compared to those treated with distilled water (5.38 ng/ml) and those who received galactogil (22.27 ng/ml). The results obtained could allow the use of the Citrullus lanatus plant through its seeds as a nutritional and lactogenic plant to combat malnutrition in infants.