To optimize the in vitro rooting conditions of M. oleifera, a study was carried out to determine the effects of exogenous auxin types on the rhizogenesis of this species. The plant material consisted of the vitro plants obtained after shoot propagation. Nodal sections with induced axillary shoots were placed for two weeks in a root induction medium consisting of MS mod 3B basal medium without auxin (control) and with three different auxins including IAA, IBA and NAA at 0.1µM. The results obtained showed that shoots were better rooted in the absence of the exogenous auxins, yielding 4.76±0.51 roots per shoot with an average length of 9.21±0.93 cm. The least rooting was obtained with NAA, which produced 2.16±0.47 roots per shoot and 3.57±0.82 cm length. NAA induced the largest calli (0.22±0.02 g) in contrast to the control (0.087±0.01 g). Thus, the auxin-free MS mod 3B basal medium would be ideal for rooting M. oleifera shoots in vitro.
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