The general objective of this research work was to assess the impact of fecal sludge discharge on the physicochemical and microbiological quality of water resources in the locality of Nomayos, a zone on the outskirts of the city of Yaoundé-Cameroon into which large quantities of raw fecal sludge from the city of Yaoundé are daily discharged. Field observations, household surveys and interviews with the emptying operators was used. Quantification and characterization of the fecal sludge collected at the discharge site and laboratory analysis of 08 groundwater samples and 06 water samples taken from the Avo'o watercourse were conducted.The average fecal coliform contents of irrigation points for vegetable crops ranged from 1012 ± 613 - 32 x 103 ± 12.80 x 102 CFU /100 mL for S0, S1, S2, and S3. The mean fecal streptococcal values for the same points are 264.20 ± 189.52 - 15.40 x 102 ± 14.20 x 102 CFU /100 mL for S0, S1, S2, S3. The average helminth egg concentrations for the different sites show that the helminth egg concentrations moved linearly to Site 3 where they reach their optimum. Then they decrease to P8 and increase progessively to P9. Sites S1, S2, S3, P8 and P9 have very high mean helminth egg concentrations of 42.33, 54.95, 71.33, 20.52 and 26.33 eggs/L respectively, compared to S0 which is 6.33. Groundwater analysis showed high concentrations of fecal pollution control germs, notably fecal coliforms and fecal streptococci.Measures for the control and protection of water resources therefore deserve to be taken in this locality for the preservation of water resources and the health of the population.