Volume 57, Issue 2, November 2021, Pages 223–228
Justine Tshidibi Tshimbila1, Hippolyte Nshimba Wa Malela2, and Benoit Dhed'a Djailo3
1 Visitor at Laboratory of Applied in Vitro Plant Biotechnology, Ghent Universit, Belgium
2 Professor, Laboratory of Botanic, Department of Botanic, Faculty of Sciences, University of Kisangani, RD Congo
3 Professor and Director of Laboratory in vitro culture Plant, Department of Biotechnology, Faculty of Sciences, University of Kisangani, RD Congo
Original language: English
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
To optimize the in vitro rooting conditions of M. oleifera, a study was carried out to determine the effects of exogenous auxin types on the rhizogenesis of this species. The plant material consisted of the vitro plants obtained after shoot propagation. Nodal sections with induced axillary shoots were placed for two weeks in a root induction medium consisting of MS mod 3B basal medium without auxin (control) and with three different auxins including IAA, IBA and NAA at 0.1µM. The results obtained showed that shoots were better rooted in the absence of the exogenous auxins, yielding 4.76±0.51 roots per shoot with an average length of 9.21±0.93 cm. The least rooting was obtained with NAA, which produced 2.16±0.47 roots per shoot and 3.57±0.82 cm length. NAA induced the largest calli (0.22±0.02 g) in contrast to the control (0.087±0.01 g). Thus, the auxin-free MS mod 3B basal medium would be ideal for rooting M. oleifera shoots in vitro.
Author Keywords: Exogenous auxins, rhizogenesis, Moringa oleifera, micropropagation, explants.
Justine Tshidibi Tshimbila1, Hippolyte Nshimba Wa Malela2, and Benoit Dhed'a Djailo3
1 Visitor at Laboratory of Applied in Vitro Plant Biotechnology, Ghent Universit, Belgium
2 Professor, Laboratory of Botanic, Department of Botanic, Faculty of Sciences, University of Kisangani, RD Congo
3 Professor and Director of Laboratory in vitro culture Plant, Department of Biotechnology, Faculty of Sciences, University of Kisangani, RD Congo
Original language: English
Copyright © 2021 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To optimize the in vitro rooting conditions of M. oleifera, a study was carried out to determine the effects of exogenous auxin types on the rhizogenesis of this species. The plant material consisted of the vitro plants obtained after shoot propagation. Nodal sections with induced axillary shoots were placed for two weeks in a root induction medium consisting of MS mod 3B basal medium without auxin (control) and with three different auxins including IAA, IBA and NAA at 0.1µM. The results obtained showed that shoots were better rooted in the absence of the exogenous auxins, yielding 4.76±0.51 roots per shoot with an average length of 9.21±0.93 cm. The least rooting was obtained with NAA, which produced 2.16±0.47 roots per shoot and 3.57±0.82 cm length. NAA induced the largest calli (0.22±0.02 g) in contrast to the control (0.087±0.01 g). Thus, the auxin-free MS mod 3B basal medium would be ideal for rooting M. oleifera shoots in vitro.
Author Keywords: Exogenous auxins, rhizogenesis, Moringa oleifera, micropropagation, explants.
How to Cite this Article
Justine Tshidibi Tshimbila, Hippolyte Nshimba Wa Malela, and Benoit Dhed'a Djailo, “Optimization trial of rooting conditions of Moringa oleifera Lam. in vitro culture,” International Journal of Innovation and Scientific Research, vol. 57, no. 2, pp. 223–228, November 2021.
