[ Evaluation in vitro de l’activité antifongique des extraits aqueux et éthanoliques de Terminalia ivorensis et de Mancozan 80 WP (fongicide de synthèse) sur Fusarium oxysporum ]
Volume 48, Issue 1, April 2020, Pages 12–20
Ruffin Kouakou Angaman1, Bernadine Marie Arobia Bosson Orsot2, Djeneb CAMARA3, Kouabenan Abo4, and Noël Guédé Zirihi5
1 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
2 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
3 Laboratoire de Botanique ; UFR Biosciences ; Université Félix HOUPHOUËT-BOIGNY, 22 BP 582 Abidjan 22, Côte d’Ivoire
4 Laboratoire de Phytopathologie et de Biologie Végétale, Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), BP 1313 Yamoussoukro, Côte d’Ivoire
5 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
Original language: French
Copyright © 2020 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
As part of the search for natural substances of plant origin as an alternative to chemical pesticides in agriculture, Terminalia ivorensis, to which antifungal virtues are lent, was used in this study to evaluate in vitro the sensitivity of Fusarium oxysporum. The antifungal activity was evaluated by the double dilution method on PDA medium with aqueous and ethanolic extracts at 70% leaf and bark of Terminalia ivorensis compared to Mancozan 80 WP which is a widely used reference fungicide. According to the results, only 70% ethanolic extracts have fungicidal properties on Fusarium oxysporum. The ethanolic bark extract of Terminalia ivorensis was fungicidal at CMF = 6.25 mg / mL. For ethanolic leaf extracts, the CMF was 12.50 mg / mL. The phytochemical sorting of the various extracts showed the presence of secondary metabolites which could justify the antifungal activity of Terminalia ivorensis and its use in traditional medicine.
Author Keywords: antifungal, fungicide, metabolites, Fusarium oxysporum, Terminalia ivorensis.
Volume 48, Issue 1, April 2020, Pages 12–20
Ruffin Kouakou Angaman1, Bernadine Marie Arobia Bosson Orsot2, Djeneb CAMARA3, Kouabenan Abo4, and Noël Guédé Zirihi5
1 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
2 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
3 Laboratoire de Botanique ; UFR Biosciences ; Université Félix HOUPHOUËT-BOIGNY, 22 BP 582 Abidjan 22, Côte d’Ivoire
4 Laboratoire de Phytopathologie et de Biologie Végétale, Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), BP 1313 Yamoussoukro, Côte d’Ivoire
5 Université Félix HOUPHOUET BOIGNY, UFR Biosciences, Laboratoire de Botanique, 22 BP 582 Abidjan 22, Côte d’Ivoire
Original language: French
Copyright © 2020 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
As part of the search for natural substances of plant origin as an alternative to chemical pesticides in agriculture, Terminalia ivorensis, to which antifungal virtues are lent, was used in this study to evaluate in vitro the sensitivity of Fusarium oxysporum. The antifungal activity was evaluated by the double dilution method on PDA medium with aqueous and ethanolic extracts at 70% leaf and bark of Terminalia ivorensis compared to Mancozan 80 WP which is a widely used reference fungicide. According to the results, only 70% ethanolic extracts have fungicidal properties on Fusarium oxysporum. The ethanolic bark extract of Terminalia ivorensis was fungicidal at CMF = 6.25 mg / mL. For ethanolic leaf extracts, the CMF was 12.50 mg / mL. The phytochemical sorting of the various extracts showed the presence of secondary metabolites which could justify the antifungal activity of Terminalia ivorensis and its use in traditional medicine.
Author Keywords: antifungal, fungicide, metabolites, Fusarium oxysporum, Terminalia ivorensis.
Abstract: (french)
Dans le cadre de la recherche de substances naturelles d’origine végétale comme alternative aux pesticides chimiques en agriculture, Terminalia ivorensis à laquelle l’on prête des vertus antifongiques a été utilisée dans cette étude pour évaluer in vitro la sensibilité de Fusarium oxysporum. L’activité antifongique a été évaluée par la méthode de double dilution sur milieu PDA avec des extraits aqueux et éthanoliques à 70% de feuilles et écorce de Terminalia ivorensis comparativement au Mancozan 80 WP qui est un fongicide de référence très utilisé.Selon les resultats, seuls les extraits éthanoliques à 70% possèdent des propriétés fongicides sur Fusarium oxysporum. L’extrait éthanolique d’écorce de Terminalia ivorensis a été fongicide à CMF = 6,25 mg/mL. Pour les extraits éthanoliques des feuilles, la CMF a été de 12,50 mg/mL. Le Tri phytochimique des différents extraits a montré la présence des métabolites secondaires qui pourraient justifier l’activité antifongique de Terminalia ivorensis et son utilisation en médecine traditionnelle.
Author Keywords: antifongiques, fongicide, métabolites, Fusarium oxysporum, Terminalia ivorensis.
How to Cite this Article
Ruffin Kouakou Angaman, Bernadine Marie Arobia Bosson Orsot, Djeneb CAMARA, Kouabenan Abo, and Noël Guédé Zirihi, “In vitro evaluation of the antifungal activity of aqueous and ethanolic extracts of Terminalia ivorensis and Mancozan 80 WP (synthetic fungicide) on Fusarium oxysporum,” International Journal of Innovation and Scientific Research, vol. 48, no. 1, pp. 12–20, April 2020.
