[ PATHOGENIE COMPAREE DE VERTICILLIUM DAHLIAE VIS–A–VIS DE DEUX VARIETES DE FRAISIER LARGEMENT CULTIVEES AU MAROC ]
Volume 21, Issue 2, April 2016, Pages 255–264
Hanane El Kaissoumi1, W. Khirallah2, Amina Ouazzani Touhami3, Mohamed Chliyeh4, Rachid Benkirane5, Karima Selmaoui6, and Allal Douira7
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofail, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
6 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
7 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The plants of two strawberry varieties ‘Fortuna and Festival’ were inoculated with two isolates of Verticillium dahliae ‘VFF and VDS’, isolated from strawberry fields lacated in the Gharb region (Morocco). The monitoring of the symptoms evolution of the Verticillium wilt, in fuction of time, on the inoculated plants has allowed to note different types of symptoms: Early yellowing followed by necrosis, stunting, wilting and death of plants especially in plants of the Fortuna variety. Calculated severity indices and the coefficients of infection, seven days after inoculation of strawberry plants are respectively about 13.5 and 11.5%. After four weeks, they reached respectively 66 and 86% for plants of the variety 'Fortuna' and 31.45 and 44.62% for those of variety 'Festival'. After six weeks, the severity indices and the coefficient of infection are respectively 77.44 and 106.8% in the 'Fortuna' plants inoculated with 'VFF' isolate. Comparisons between indices of severity and coefficients infection in inoculated plants of both varieties have shown that this is the variety 'Fortuna' which is most sensitive to isolate 'VFF', which showed pathogen also against the 'Festival' variety. Both tested isolates 'VFF and VFS' were re-isolated from all levels of plants: roots, collars and leaf petioles.
Author Keywords: Morocco, Strawberry, inoculation, Verticillium dahliae, wilting, pathogenicity.
Volume 21, Issue 2, April 2016, Pages 255–264
Hanane El Kaissoumi1, W. Khirallah2, Amina Ouazzani Touhami3, Mohamed Chliyeh4, Rachid Benkirane5, Karima Selmaoui6, and Allal Douira7
1 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
2 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
3 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
4 Université Ibn Tofail, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
5 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
6 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
7 Université Ibn Tofaïl, Faculté des Sciences, Laboratoire de Botanique et de Protection des Plantes, B.P. 133, Kenitra, Morocco
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The plants of two strawberry varieties ‘Fortuna and Festival’ were inoculated with two isolates of Verticillium dahliae ‘VFF and VDS’, isolated from strawberry fields lacated in the Gharb region (Morocco). The monitoring of the symptoms evolution of the Verticillium wilt, in fuction of time, on the inoculated plants has allowed to note different types of symptoms: Early yellowing followed by necrosis, stunting, wilting and death of plants especially in plants of the Fortuna variety. Calculated severity indices and the coefficients of infection, seven days after inoculation of strawberry plants are respectively about 13.5 and 11.5%. After four weeks, they reached respectively 66 and 86% for plants of the variety 'Fortuna' and 31.45 and 44.62% for those of variety 'Festival'. After six weeks, the severity indices and the coefficient of infection are respectively 77.44 and 106.8% in the 'Fortuna' plants inoculated with 'VFF' isolate. Comparisons between indices of severity and coefficients infection in inoculated plants of both varieties have shown that this is the variety 'Fortuna' which is most sensitive to isolate 'VFF', which showed pathogen also against the 'Festival' variety. Both tested isolates 'VFF and VFS' were re-isolated from all levels of plants: roots, collars and leaf petioles.
Author Keywords: Morocco, Strawberry, inoculation, Verticillium dahliae, wilting, pathogenicity.
Abstract: (french)
Les plants de deux variétés de fraisier ‘Fortuna et Festival’ ont été inoculés par deux isolats de Verticillium dahliae ‘VFF et VFS’, isolés des fraiseraies de la région du Gharb (Maroc). Le suivi de l’évolution des symptômes de la verticilliose, en fonction du temps, chez les plants de fraisier inoculés a permis de noter différents types de symptômes: jaunissements précoces suivis de nécroses, nanisme, flétrissement et mort des plants surtout chez les plants de la variété Fortuna. Les indices de sévérité et les coefficients d’infection calculés, sept jours après inoculation des plants de fraisier, sont respectivement de l’ordre de 13,5 et 11,5%. Après 4 semaines, ils atteignent respectivement 66 et 86% chez les plants de la variété ‘Fortuna’ et 31,45 et 44,62% chez ceux de la variété ‘Festival’. Après six semaines, l’indice de sévérité et le coefficient d’infection sont respectivement de 77,44 et 106,8% chez les plants de ‘Fortuna ‘ inoculés par l’isolat ‘VFF’. Les comparaisons entre les indices de sévérité et les coefficients d’infection chez les plants inoculés des deux variétés ont montré que c’est la variété ‘Fortuna’ qui est la plus sensible à l’isolat ‘VFF’, isolat qui s’est montré également pathogène vis-à-vis de la variété ‘Festival’. Les deux isolats testés ‘VFF et VFS’ ont été réisolés de tous les niveaux des plants : racines, collets et pétioles des feuilles.
Author Keywords: Maroc, fraisier, inoculation, Verticillium dahliae, verticilliose, pouvoir pathogène.
How to Cite this Article
Hanane El Kaissoumi, W. Khirallah, Amina Ouazzani Touhami, Mohamed Chliyeh, Rachid Benkirane, Karima Selmaoui, and Allal Douira, “Comparative pathogenicity of Verticillium dahliae against two strawberry plant varieties widely cultivated in Morocco,” International Journal of Innovation and Scientific Research, vol. 21, no. 2, pp. 255–264, April 2016.
