[ Effet de quelques facteurs physico-chimiques sur la gynogenèse in vitro de trois variétés de Blé dur ]
Volume 14, Issue 2, April 2015, Pages 168–177
Sonia Mansouri1, Leila Radhouane2, Hugues Nzeingui3, and Sadok Bouzid4
1 Institut National de la Recherche Agronomique de Tunis, Ariana 2049, Tunisia
2 Tunisian Institute for Agriculture Research (INRAT), Hédi Karray Street, Ariana 2049, Tunisia
3 ENSAIA/INPL, 54505, France
4 Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunisia
Original language: French
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Use of doubled haploïdes allows to shorten considerably the cycle of classic selection and to obtain quickly a stable homozygotie. However, regeneration of albino plants, particularly for cereal, constitutes the major problem of this approach. Obtaining haplodiploïdes by in vitro culture of not fertilized ovaries is determined by numerous factors biological as physical or chemical. Our work concerned the optimization of the answer gynogenetique of three varieties of durum wheat (Cocorit, Isly, Jori) cultivated on two different media (M1 et M2) and subjected to a cold pre-treatment at 4 ° C for 7, 11 and 15 days.This work has demonstrated that genotypes interact strongly with pre-treatment duration and with induction medium composition. Thus, a beneficial effect of thermal pre-treatment was noted on the induction of embryos and production of callus, and also on the quality of regenerating. For the regeneration of green plants, the response of genotypes varied with the induction medium composition and with cold pre-treatment period. Indeed, M1 medium was favourable for Cocorit and Jori varieties but with different incubation duration. For cons, M2 medium gave better results with Isly genotype for 7 days incubation.
Author Keywords: Breeding, Triticum durum, Doubled haploid plant production, Callus, Tunisia.
Volume 14, Issue 2, April 2015, Pages 168–177
Sonia Mansouri1, Leila Radhouane2, Hugues Nzeingui3, and Sadok Bouzid4
1 Institut National de la Recherche Agronomique de Tunis, Ariana 2049, Tunisia
2 Tunisian Institute for Agriculture Research (INRAT), Hédi Karray Street, Ariana 2049, Tunisia
3 ENSAIA/INPL, 54505, France
4 Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunisia
Original language: French
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Use of doubled haploïdes allows to shorten considerably the cycle of classic selection and to obtain quickly a stable homozygotie. However, regeneration of albino plants, particularly for cereal, constitutes the major problem of this approach. Obtaining haplodiploïdes by in vitro culture of not fertilized ovaries is determined by numerous factors biological as physical or chemical. Our work concerned the optimization of the answer gynogenetique of three varieties of durum wheat (Cocorit, Isly, Jori) cultivated on two different media (M1 et M2) and subjected to a cold pre-treatment at 4 ° C for 7, 11 and 15 days.This work has demonstrated that genotypes interact strongly with pre-treatment duration and with induction medium composition. Thus, a beneficial effect of thermal pre-treatment was noted on the induction of embryos and production of callus, and also on the quality of regenerating. For the regeneration of green plants, the response of genotypes varied with the induction medium composition and with cold pre-treatment period. Indeed, M1 medium was favourable for Cocorit and Jori varieties but with different incubation duration. For cons, M2 medium gave better results with Isly genotype for 7 days incubation.
Author Keywords: Breeding, Triticum durum, Doubled haploid plant production, Callus, Tunisia.
Abstract: (french)
L'utilisation des lignées haploïdes doublées permet de raccourcir considérablement le cycle de sélection classique et d'obtenir rapidement une homozygotie stable. Toutefois, la régénération de plantes albinos, particulièrement chez les céréales, constitue le problème majeur de cette approche. La technique d'obtention des haplodiploïdes par une culture in vitro d'ovaires non fécondés est déterminée par de nombreux facteurs aussi bien biologiques que physiques ou chimiques. Notre travail a porté sur l'optimisation de la réponse gynogénétique de trois variétés de blé dur (Cocorit, Isly, Jori) cultivées sur deux milieux différents (M1 et M2) et soumises à un prétraitement au froid à 4°C pendant 7, 11 et 15 jours.
Ce travail a permis de montrer que les génotypes interagissent fortement avec la durée de prétraitement et avec la composition du milieu d'induction. Ainsi, un effet bénéfique du prétraitement thermique a été noté sur l'induction d'embryons et la production de cals, et aussi, sur la qualité des régénérants. Pour la régénération de plantes chlorophylliennes, la réponse des génotypes a varié avec la composition du milieu d'induction et la durée de prétraitement au froid. En effet, le milieu M1 a été favorable aux variétés Cocorit et Jori mais avec des durées d'incubation différentes. Par contre, le milieu M2 a donné de meilleurs résultats avec le génotype Isly pour une durée de 7jours.
Author Keywords: Amélioration génétique, Blé dur, Haplodiploïdisation, Cal, Tunisie.
How to Cite this Article
Sonia Mansouri, Leila Radhouane, Hugues Nzeingui, and Sadok Bouzid, “Effect of some physico-chemical factors on in vitro gynogenesis of three varieties of durum wheat,” International Journal of Innovation and Scientific Research, vol. 14, no. 2, pp. 168–177, April 2015.
