Volume 16, Issue 2, July 2015, Pages 526–532
Okafor Collins Onyebuchi Okeke1, Iroha Ifeanyichukwu Romanus2, Nwadiogbu Ifeanyi Anthony3, Agumah Nnabuife4, Eni Obehi5, and Odinkemere Stephen Chijindu6
1 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
2 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
3 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
4 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
5 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
6 Department of Dialysis, Abia State Specialist Hospital & Diagnostic Centre, Umuahia, Nigeria
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study investigated the susceptibility pattern of coliform bacteria isolated from sachet and borehole waters sold in Abakaliki metropolis. This was done by determining the presence of Escherichia coli, Klebsiella spp and Enterobacter spp and their antibiotic susceptibility profile using commercially prepared antibiotic discs. A total of Five hundred (500) samples of water comprising 250 each from selected brands of sachet water and boreholes were obtained from water dispensers in Abakaliki metropolis, Ebonyi State, Nigeria. Results of biochemical analysis revealed that out of the 311 confirmed isolates, 138 (55.2%) were from sachet waters while 173 (69%) were from borehole waters. The susceptibility profile of the isolates to ten antimicrobial agents indicated that majority of the isolates showed little susceptibility and was highly resistant to the following antimicrobial agents (nitrofurantoin, amoxycillin, ampicillin, ciprofloxacin, tetracycline, norbactin/norfloxacin, ofloxacin, cefuroxime and gentamicin). This showed that they exhibit multi-drug resistance pattern which is a common feature of medically important coliform bacteria. None of the water sources met the WHO microbiological standards for drinking water and thus pose a serious health risk to its consumers and users if not properly treated. We therefore report the presence of multi-drug resistant coliform bacteria in sachet and borehole waters sold in Abakaliki metropolis, Ebonyi State, Nigeria.
Author Keywords: Microbiological standard, coliform, Bacteria, sucesptibility, sachet water, borehole water, Abakaliki.
Okafor Collins Onyebuchi Okeke1, Iroha Ifeanyichukwu Romanus2, Nwadiogbu Ifeanyi Anthony3, Agumah Nnabuife4, Eni Obehi5, and Odinkemere Stephen Chijindu6
1 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
2 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
3 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
4 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
5 Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
6 Department of Dialysis, Abia State Specialist Hospital & Diagnostic Centre, Umuahia, Nigeria
Original language: English
Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This study investigated the susceptibility pattern of coliform bacteria isolated from sachet and borehole waters sold in Abakaliki metropolis. This was done by determining the presence of Escherichia coli, Klebsiella spp and Enterobacter spp and their antibiotic susceptibility profile using commercially prepared antibiotic discs. A total of Five hundred (500) samples of water comprising 250 each from selected brands of sachet water and boreholes were obtained from water dispensers in Abakaliki metropolis, Ebonyi State, Nigeria. Results of biochemical analysis revealed that out of the 311 confirmed isolates, 138 (55.2%) were from sachet waters while 173 (69%) were from borehole waters. The susceptibility profile of the isolates to ten antimicrobial agents indicated that majority of the isolates showed little susceptibility and was highly resistant to the following antimicrobial agents (nitrofurantoin, amoxycillin, ampicillin, ciprofloxacin, tetracycline, norbactin/norfloxacin, ofloxacin, cefuroxime and gentamicin). This showed that they exhibit multi-drug resistance pattern which is a common feature of medically important coliform bacteria. None of the water sources met the WHO microbiological standards for drinking water and thus pose a serious health risk to its consumers and users if not properly treated. We therefore report the presence of multi-drug resistant coliform bacteria in sachet and borehole waters sold in Abakaliki metropolis, Ebonyi State, Nigeria.
Author Keywords: Microbiological standard, coliform, Bacteria, sucesptibility, sachet water, borehole water, Abakaliki.
How to Cite this Article
Okafor Collins Onyebuchi Okeke, Iroha Ifeanyichukwu Romanus, Nwadiogbu Ifeanyi Anthony, Agumah Nnabuife, Eni Obehi, and Odinkemere Stephen Chijindu, “ANTIMICROBIAL RESISTANCE PATTERN OF COLIFORM BACTERIA ISOLATED FROM SACHET AND BOREHOLE WATERS SOLD IN ABAKALIKI METROPOLIS OF EBONYI STATE, NIGERIA,” International Journal of Innovation and Scientific Research, vol. 16, no. 2, pp. 526–532, July 2015.
