This study deals with the morphological features and diet of the frog Hoplobatrachus occipitalis in Benin. Specimens were caught in the River basins of Ouémé, Mono and Niger using the light fishing technique. Morphometric data were taken on 180 individuals while diet data were collected on 90 specimens per hydrographic basin. The mean values of individual weight, total length and snout-cloaca length of H. occipitalis in the Niger River basin are significantly lower compared to the other two basins (p<0.05) where the values of the three parameters are statistically similar (p>0.05). Compared to males, females displayed higher values of these parameters and most of the differences are significant in the Ouémé and Mono River basins (p<0.05). The overall emptiness index is 29.9%, the highest and lowest values being obtained in the Niger River basin (43.3 ± 2.4%) and Ouémé River basin (13.6 ± 2.5%), respectively (p<0.05). The diversity of prey in the stomachs of frogs from the Niger River basin (Insects, plant debris and grains of sand) is low compared to the Ouémé River basin (Insects, plant debris, Arachnids, Molluscs, Fish and grains of sand) and the Mono basin (Insects, plant debris, Fish and grains of sand). Insects are the most frequent, the most numerically abundant and the most weight-representative in the three River basins. They are the main food in the diet of H. occipitalis in the Ouémé and Niger River basins while in the Mono River basin, they are with the plant debris of secondary foods. All other prey encountered are incidental in the diet of H. occipitalis. These biological data are useful for the breeding of H. occipitalis in Benin.