[ Evaluation de l’effet des extraits de quelques plantes médicinales sur les souches de bactéries pathogènes entériques et de la flore intestinale par des tests antibiogrammes ]
Volume 37, Issue 2, July 2018, Pages 199–215
NTAZONGWA BUZERA Balzac1
1 Section des Sciences, Institut Supérieur Pédagogique de Baraka (ISP/Baraka), Fizi / Baraka, Sud Kivu, RD Congo
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present work is a contribution to the study of the fight against enteric diseases by traditional therapeutics. It evaluates the effects of the aqueous extracts of Ephorbia hirta, Ipomea Involucrata, Mangifera indica, Musa ensete, Oxalis corymboza and Psidium goyaya and those of organic extracts based on methanol from the barks of Mangifera indica on strains of normal flora; E. coli and Enterobacter aerogenes and pathogenic strains of Salmonella typhi, Shigella flexneri and Shigella sonnei by the antibiogram test technique. The first tests with aqueous extracts for all plants provide valuable information on empirical recipe values. Some plants commonly used in the fight against diarrhea have an antibacterial effect. This is the case of Mangifera indica (barks and leaves), Musa ensete and Psidium goyava. These extracts also have in fact the strains of E. coli or Enterobacter aerogenes which represents a danger for the normal intestinal flora. Other plants, on the other hand, have no antibacterial effect or have a very reduced effect. Thus Oxalis corymboza and Ipomea involucrata appear to be involved in other antidiarrheal mechanisms. Euphobia hirta has a specific action on E. coli but it could be said that its antidysenteric role is specific to amoebiasis. Mangifera indica barks provide the most relevant extract, acting on all strains with the exception of Enterobacter aerogenes. The aqueous extracts based on methanol obtained after dilution give, during the tests, the MIC (Minimum Inhibitory Concentration) of a few mg / ml for Shigella sonnei (10-3 dilution) of a few tenth of mg / ml for Shigella flexneri and Salmonella typhi (dilution 10-4) and some one hundredth of mg / ml for E.coli (Dilution 10-5). The MIC for E. coli confirms the empirical recipes. The hypothesis that these barks are frequently used in cases of gastroenteritis in children is verified. The antibiogram tests carried out provide details on the empirical recipes and make it possible to affirm that the barks of Magnifera indica are effective against the gastroenteritis of children; the active ingredient is soluble in methanol, some plants present a danger for normal intestinal flora hence the need to associate vitamin B complex and that other plants have no antibacterial effect.
Author Keywords: Medicinal plant, enterobacterium, antibiogram, extractive product.
Volume 37, Issue 2, July 2018, Pages 199–215
NTAZONGWA BUZERA Balzac1
1 Section des Sciences, Institut Supérieur Pédagogique de Baraka (ISP/Baraka), Fizi / Baraka, Sud Kivu, RD Congo
Original language: French
Copyright © 2018 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present work is a contribution to the study of the fight against enteric diseases by traditional therapeutics. It evaluates the effects of the aqueous extracts of Ephorbia hirta, Ipomea Involucrata, Mangifera indica, Musa ensete, Oxalis corymboza and Psidium goyaya and those of organic extracts based on methanol from the barks of Mangifera indica on strains of normal flora; E. coli and Enterobacter aerogenes and pathogenic strains of Salmonella typhi, Shigella flexneri and Shigella sonnei by the antibiogram test technique. The first tests with aqueous extracts for all plants provide valuable information on empirical recipe values. Some plants commonly used in the fight against diarrhea have an antibacterial effect. This is the case of Mangifera indica (barks and leaves), Musa ensete and Psidium goyava. These extracts also have in fact the strains of E. coli or Enterobacter aerogenes which represents a danger for the normal intestinal flora. Other plants, on the other hand, have no antibacterial effect or have a very reduced effect. Thus Oxalis corymboza and Ipomea involucrata appear to be involved in other antidiarrheal mechanisms. Euphobia hirta has a specific action on E. coli but it could be said that its antidysenteric role is specific to amoebiasis. Mangifera indica barks provide the most relevant extract, acting on all strains with the exception of Enterobacter aerogenes. The aqueous extracts based on methanol obtained after dilution give, during the tests, the MIC (Minimum Inhibitory Concentration) of a few mg / ml for Shigella sonnei (10-3 dilution) of a few tenth of mg / ml for Shigella flexneri and Salmonella typhi (dilution 10-4) and some one hundredth of mg / ml for E.coli (Dilution 10-5). The MIC for E. coli confirms the empirical recipes. The hypothesis that these barks are frequently used in cases of gastroenteritis in children is verified. The antibiogram tests carried out provide details on the empirical recipes and make it possible to affirm that the barks of Magnifera indica are effective against the gastroenteritis of children; the active ingredient is soluble in methanol, some plants present a danger for normal intestinal flora hence the need to associate vitamin B complex and that other plants have no antibacterial effect.
Author Keywords: Medicinal plant, enterobacterium, antibiogram, extractive product.
Abstract: (french)
Le présent travail est une contribution à l’étude de la lutte contre les maladies entériques par la thérapeutique traditionnelle. Il évalue les effets des extraits aqueux d’Ephorbia hirta, Ipomea Involucrata, Mangifera indica, Musa ensete, Oxalis corymboza et Psidium goyaya et ceux des extraits organiques à base de méthanol des écorces de Mangifera indica sur les souches de la flore normale ; E.coli et Enterobacter aerogenes et sur les souches pathogènes de Salmonella typhi, Shigella flexneri et Shigella sonnei par la technique des tests antibiogrammes. Les premiers tests effectués avec des extraits aqueux pour l’ensemble des plantes fournissent de précieux renseignements sur les valeurs de recettes empiriques. Certaines plantes utilisées couramment dans la lutte contre les diarrhées ont un effet antibactérien. Tel est le cas de Mangifera indica (écorces et feuilles), Musa ensente et Psidium goyava. Ces extraits ont aussi en effet sur les souches d’E.coli ou d’Enterobacter aerogenes, ce qui représente un danger pour la flore normale intestinale. D’autres plantes par contre n’ont aucun effet antibactérien ou présentent un effet très réduit. Ainsi Oxalis corymnoza et Ipomea involucrata semble intervenir dans d’autres mécanisme antidiarrhétiques. Euphobia hirta présente une action spécifique sur E. coli mais on pourrait affirmer que son rôle antidysentérique est propre à l’amibiase. Les écorces de Mangifera indica fournissent l’extrait le plus intéressent, agissant sur toutes les souches à l’exception d’Enterobacter aerogenes. Les extraits aqueux à base de méthanol obtenus après dilution donnent lors des tests les CMI (Concentration Minimale Inhibitrice) de quelques mg/ml pour Shigella sonnei (dilution 10-3) de quelques dixième de mg/ml pour Shigella flexneri et Salmonella typhi (dilution 10-4) et de quelque centième de mg/ml pour E.coli (Dilution 10-5). La CMI pour E.coli confirme les recettes empiriques. L’hypothèse selon laquelle ces écorces sont fréquemment employées en cas de gastro-entérites des enfants est vérifiée. Les tests antibiogrammes réalisés fournissent des précisions sur les recettes empiriques et permettent d’affirmer que les écorces de Magnifera indica sont efficaces contre les gastro-entérites des enfants ; le principe actif est soluble dans le méthanol, certaines plantes présentent un danger pour la flore normale intestinale d’où la nécessité d’associer des vitamines B complexe et que d’autres plantes n’ont aucun effet antibactérien.
Author Keywords: Plante médicinale, entérobactérie, antibiogramme, produit d’extraction.
How to Cite this Article
NTAZONGWA BUZERA Balzac, “Evaluation of the effect of extracts of some medicinal plants on strains of enteric pathogenic bacteria and intestinal flora by antibiogram tests,” International Journal of Innovation and Scientific Research, vol. 37, no. 2, pp. 199–215, July 2018.
