[ Estimation du niveau de connaissance et Analyse toxicologique chez des manipulateurs de pesticides organophosphorés exposés au Fénitrothion dans la région de Sfax, en Tunisie ]
Volume 25, Issue 1, June 2016, Pages 199–211
Asma Ghorbel1, Houda LAZREG AREF2, Mounira Hajjeji Darouiche3, Neila Moalla Nouri4, Mohamed Laarbi Masmoudi5, and Feriele Messadi Akrout6
1 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
2 Biochemistry Laboratory, LR12ES05 Nutrition- Functional Foods and vascular Health, Faculty of Medicine, Monastir, Tunisia
3 Working Medicine Service, Hedi Chaker Hospital, Sfax, Tunisia
4 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
5 Working Medicine Service, Hedi Chaker Hospital, Sfax, Tunisia
6 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Organophosphate pesticides, commonly used in the occupational field, represent a real threat to human health and the environment. The aim of our study is to estimate the level of knowledge of the subjects exposed to these products and assess their toxicity biomarkers. We conducted a prospective descriptive survey about 26 pesticide handlers who checked in occupational medicine service in Hedi Chaker university hospital. A questionnaire allowed us to gather information about the level of workers education and their practices of organophosphate pesticides spacifically Fenitrothion. Three samples of blood and urine tests were collected before and after exposure and after rest in hygiene service. Each blood sample was used for the determination of serum and erythrocyte cholinesterase activity. The detection of organophosphate pesticides was made in the urine by GC/MS. A low level of knowledge of the manipulators for pesticides (global score was 20.46) was found. Reference globular cholinesterase activities were all below the usual values. Serum cholinesterase activities were decreased by 30 to 50% in 48% of patients and more than 50% in 30% of the subjects. These activities have recovered after rest. The detection of pesticides (fenitrothion) was positive in 4 workers. Biological assays show impregnation of manipulators by organophosphate. Their kinetic reflects the exposure of subjects with the pesticide. These data were correlated with the low level of workers knowledge (p=0.01).
Author Keywords: Organophosphorus, Pesticides, Cholinesterases, fenitrothion, site esterasique, biomarqueurs.
Volume 25, Issue 1, June 2016, Pages 199–211
Asma Ghorbel1, Houda LAZREG AREF2, Mounira Hajjeji Darouiche3, Neila Moalla Nouri4, Mohamed Laarbi Masmoudi5, and Feriele Messadi Akrout6
1 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
2 Biochemistry Laboratory, LR12ES05 Nutrition- Functional Foods and vascular Health, Faculty of Medicine, Monastir, Tunisia
3 Working Medicine Service, Hedi Chaker Hospital, Sfax, Tunisia
4 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
5 Working Medicine Service, Hedi Chaker Hospital, Sfax, Tunisia
6 Hygiene Laboratory, Hedi Chaker Hospital, Sfax, Tunisia
Original language: French
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Organophosphate pesticides, commonly used in the occupational field, represent a real threat to human health and the environment. The aim of our study is to estimate the level of knowledge of the subjects exposed to these products and assess their toxicity biomarkers. We conducted a prospective descriptive survey about 26 pesticide handlers who checked in occupational medicine service in Hedi Chaker university hospital. A questionnaire allowed us to gather information about the level of workers education and their practices of organophosphate pesticides spacifically Fenitrothion. Three samples of blood and urine tests were collected before and after exposure and after rest in hygiene service. Each blood sample was used for the determination of serum and erythrocyte cholinesterase activity. The detection of organophosphate pesticides was made in the urine by GC/MS. A low level of knowledge of the manipulators for pesticides (global score was 20.46) was found. Reference globular cholinesterase activities were all below the usual values. Serum cholinesterase activities were decreased by 30 to 50% in 48% of patients and more than 50% in 30% of the subjects. These activities have recovered after rest. The detection of pesticides (fenitrothion) was positive in 4 workers. Biological assays show impregnation of manipulators by organophosphate. Their kinetic reflects the exposure of subjects with the pesticide. These data were correlated with the low level of workers knowledge (p=0.01).
Author Keywords: Organophosphorus, Pesticides, Cholinesterases, fenitrothion, site esterasique, biomarqueurs.
Abstract: (french)
Les pesticides organophosphorés (OP), fréquemment utilisés en milieu professionnel, représentent une menace réelle pour la santé de l’Homme et pour l’environnement. L’objectif de notre étude est d’estimer le niveau de connaissance des sujets exposés à ces produits et évaluer leur toxicité par des marqueurs biologiques. Nous avons mené une enquête prospective descriptive à propos de 26 manipulateurs de pesticides ayant consulté au service de médecine de travail au CHU Hédi Chaker de Sfax. Un questionnaire nous a permis de collecter les informations concernant le niveau d’instruction des ouvriers ainsi que leur manipulation de pesticides organophosphorés et spécifiquement le fénitrothion. Trois prélèvements de sang et d’urine ont été réalisés, avant et après exposition puis après repos. Chaque prélèvement sanguin a servi pour le dosage des activités cholinestérasiques sérique et globulaire. La détection de pesticides organophosphorés a été faite au niveau des urines à l’aide de la GC/MS. Le niveau de connaissance des manipulateurs concernant les pesticides était faible (score global moyen : 20,46 sur 43). Les activités cholinestérasiques globulaires de référence étaient toutes inférieures aux valeurs usuelles. Les activités cholinestérasiques sériques ont baissé de 30 à 50% chez 48% des sujets et de plus de 50% chez 30% des sujets. Ces activités se sont rétablies après repos. Leur cinétique reflète bien l’exposition des sujets avec le pesticide. La détection de pesticides (fénitrothion) était positive chez 4 travailleurs. Ces données ont été corrélées avec le faible niveau de connaissances des travailleurs (p=0,01).
Author Keywords: .
How to Cite this Article
Asma Ghorbel, Houda LAZREG AREF, Mounira Hajjeji Darouiche, Neila Moalla Nouri, Mohamed Laarbi Masmoudi, and Feriele Messadi Akrout, “Estimated level of knowledge and Toxicological analysis in organophosphate pesticide handlers in the region of Sfax,” International Journal of Innovation and Scientific Research, vol. 25, no. 1, pp. 199–211, June 2016.
