Volume 23, Issue 1, May 2016, Pages 1–9
J Pieter H van Wyk1 and J Boitumelo M Sibiya2
1 Department of Pharmacology and Therapeutics, Sefako Makgatho Health Sciences University, Ga-Rankuwa, South Africa
2 Department of Pharmacology and Therapeutics, Sefako Makgatho Health Sciences University, Ga-Rankuwa, South Africa
Original language: English
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Waste office paper is a major component of solid waste of which thousands of tons are produced daily. The cellulose section of waste office paper can be hydrolyzed by cellulase enzymes into glucose a fermentable sugar. Relative high and low cellulase concentrations from Aspergillus niger have been used to degrade waste office paper that was 100% and 50% covered with ink. Office paper free of ink was also exposed to these enzyme concentrations at incubation temperatures of 300C, 400C, 500C and 600C. The ink free office paper showed the highest degree of sugar formation at a concentration of 23 mg.ml-1 during an incubation at 500C and when treated with the highest enzyme concentration. The highest amount of sugar (18 mg.ml-1) produced from office paper 50 % covered with ink when exposed to the high cellulase concentration was obtained at 400C whilst an incubation at the same temperature resulted in the paper 100 % covered with ink to be maximally degraded producing a sugar concentration of 17 mg.ml-1. When exposed to the lower enzyme concentration maximum bioconversion of all office paper materials (100 % as well as 50 % covered with ink and ink free) was obtained at 400C with sugar produced at concentrations between 2,8 and 6,6 mg.ml-1.
Author Keywords: Waste office paper, ink, saccharification, Aspergillus niger, efficiency, cellulose.
J Pieter H van Wyk1 and J Boitumelo M Sibiya2
1 Department of Pharmacology and Therapeutics, Sefako Makgatho Health Sciences University, Ga-Rankuwa, South Africa
2 Department of Pharmacology and Therapeutics, Sefako Makgatho Health Sciences University, Ga-Rankuwa, South Africa
Original language: English
Copyright © 2016 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Waste office paper is a major component of solid waste of which thousands of tons are produced daily. The cellulose section of waste office paper can be hydrolyzed by cellulase enzymes into glucose a fermentable sugar. Relative high and low cellulase concentrations from Aspergillus niger have been used to degrade waste office paper that was 100% and 50% covered with ink. Office paper free of ink was also exposed to these enzyme concentrations at incubation temperatures of 300C, 400C, 500C and 600C. The ink free office paper showed the highest degree of sugar formation at a concentration of 23 mg.ml-1 during an incubation at 500C and when treated with the highest enzyme concentration. The highest amount of sugar (18 mg.ml-1) produced from office paper 50 % covered with ink when exposed to the high cellulase concentration was obtained at 400C whilst an incubation at the same temperature resulted in the paper 100 % covered with ink to be maximally degraded producing a sugar concentration of 17 mg.ml-1. When exposed to the lower enzyme concentration maximum bioconversion of all office paper materials (100 % as well as 50 % covered with ink and ink free) was obtained at 400C with sugar produced at concentrations between 2,8 and 6,6 mg.ml-1.
Author Keywords: Waste office paper, ink, saccharification, Aspergillus niger, efficiency, cellulose.
How to Cite this Article
J Pieter H van Wyk and J Boitumelo M Sibiya, “Relative and efficient saccharification of waste office paper by different concentrations of Aspergillus niger cellulase at various temperatures,” International Journal of Innovation and Scientific Research, vol. 23, no. 1, pp. 1–9, May 2016.
